- Title
- Molecular epidemiology of methicillin-resistant staphylococcus aureus isolates in New South Wales, Australia, 2012-2017
- Creator
- Dotel, Ravindra; O'Sullivan, Matthew V. N.; Davis, Joshua S.; Newton, Peter J.; Gilbert, Gwendolyn L.
- Relation
- NHMRC.1010452 http://purl.org/au-research/grants/nhmrc/1010452
- Relation
- Infection, Disease and Health Vol. 24, Issue 3, p. 134-140
- Publisher Link
- http://dx.doi.org/10.1016/j.idh.2019.04.002
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2019
- Description
- Background: To better understand the molecular epidemiology of MRSA and to assess the utility of 19-target binary typing we undertook large-scale epidemiological surveillance of MRSA from invasive and non-invasive clinical specimens, and screening swabs. Methods: Binary typing was performed on clinical MRSA isolates collected in New South Wales (NSW), Australia between 01/01/2012 - 31/12/2017. Binary type (BT) predicted multilocus sequence type (ST) and spa types based on results from isolates which had been characterised by both methods. Results: 7624 MRSA isolates were analysed of which 3581 (47%) were wounds or skin & softtissue isolates (W/SSTI), 2436 (32%) screening swabs, 469 (6%) blood cultures (BC), 780 (10%) others, and 358 (5%) unknown. We identified 731 BTs, 54 spa types, and 31 STs. ST239 was the commonest MRSA clone in 2012 (30%), but it decreased to 7% in 2017 (p <0.001). In contrast, <0.5% of MRSA were ST45 in 2012 compared to 14% in 2017 (p<0.001). An emergence of PVL-positive ST22 was also noted. Of all isolates, 28% (2122/7624) were lukS/PVL positive; the proportion, among prospectively collected isolates increased from 24% (1406/5858) to 33% (1933/5858) between 2012 and 2017 (p <0.0001). 43% (1534/3581) W/SSTI, 20% (95/469) BC and 10% (239/2436) screening swabs were PVL-positive. Conclusions: A major change in the epidemiology of MRSA was noted with a decline of ST239, an emergence of ST45 and PVL-positive ST22, and a significant increase in PVL-positive isolates. Binary typing can be a useful routine laboratory test for prospective molecular surveillance of MRSA colonisation and infection.
- Subject
- prospective studies; leukocidins; staphylococcal infections; blood culture; molecular epidemiology; MRSA; SDG 3; Sustainable Development Goals
- Identifier
- http://hdl.handle.net/1959.13/1469887
- Identifier
- uon:48332
- Identifier
- ISSN:2468-0451
- Language
- eng
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